Rapid Quantification of Gene Expression by Competitive RT-PCR and Ion-Pair Reversed-Phase HPLC
Competitive reverse-transcription PCR (RT-PCR) techniques for quantification of gene expression employ titrations in which the products of multiple PCRs must be separated, analyzed tonies jj audio play character from cocomelon and quantified to compute gene expression in a single sample.We have employed a novel, ion-pair reversed-phase HPLC (IP-RP-